pathogenic bacteria escherichia coli o157 h7  (ATCC)

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Characterisation of elemental composition and spectra of Chitligsan. (A) Constituent elements and EDS image patterns, (B) XRF results, and (C) FTIR spectra of Chitligsan.

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques:

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Analysis of the XRD patterns of Chitligsan. Calcite was the most abundant mineral (42.8%), followed by hydrotalcite (40.3%), clinopyroxene 15.6%, and ilmenite 1.3%.

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques:

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Gas chromatography of volatile compounds generated from pyrolysis of chitosan standard (black trace) and Chitligsan (blue trace).

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques: Gas Chromatography, Generated

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Proposed formation mechanism of Chitligsan. Desert sand minerals (Fe 2 O 3 , FeOOH) interact with oxalic acid and light, promoting Fe( iii )/Fe( ii ) redox cycling through chelation and partial reduction under light and moisture, leading to the formation of the mineral composite Chitligsan.

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques:

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques: Suspension, Control

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: SEM micrographs illustrating the morphological changes of S. aureus (A–C) and E. coli (D–F) upon exposure to Chitligsan or citric acid. (A and D) Untreated control cells; (B and E) cells treated with 0.1 M citric acid; (C and F) cells treated with Chitligsan (1 mg mL −1 ) and 0.1 M citric acid.

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques: Control

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: MTT assay results for L929 cells after 24 h of exposure to serial dilutions (50 mg mL −1 , 25 mg mL −1 , 12.5 mg mL −1 , 6.25 mg mL −1 ) of Chitligsan stock solution (100 mg mL −1 ). N = 3, * p < 0.05; ** p < 0.01. Control: untreated cells. Positive control (cytotoxic agent): 2.5% DMSO.

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques: MTT Assay, Control, Positive Control

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Scratch assay results for L929 cells after 48 h of exposure to Chitligsan (100 mg mL −1 ). Quantification of scratch closure at 24 h and 48 h relative to 0 h in control and Chitligsan-treated groups. The scale bar in the images represents 100 µm. Control: untreated cells. Quantitative analysis of scratch closure (%) at 24 h and 48 h relative to 0 h. (A) Quantification of scratch closure at 24 h and 48 h relative to 0 h, (B) Representative phase-contrast images of scratch wounds at 0 h, 24 h, and 48 h.

Article Snippet: In addition to physicochemical examination, the antibacterial activity of Chitligsan against pathogenic bacteria ( Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213), alongside its cytotoxicity and impact on cell viability, was evaluated to gain further insight into its compatibility with biological systems.

Techniques: Wound Healing Assay, Control